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normal colon mucosal epithelial cell line ncm460  (China Center for Type Culture Collection)

 
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    China Center for Type Culture Collection normal colon mucosal epithelial cell line ncm460
    Normal Colon Mucosal Epithelial Cell Line Ncm460, supplied by China Center for Type Culture Collection, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/normal+colon+mucosal+epithelial+cell+line+ncm460/ncm+460/pm36741232-101-0-11
    Average 90 stars, based on 1 article reviews
    normal colon mucosal epithelial cell line ncm460 - by Bioz Stars, 2026-11
    90/100 stars

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    Article Title: Pyroptosis-Related Signature Predicts the Progression of Ulcerative Colitis and Colitis-Associated Colorectal Cancer as well as the Anti-TNF Therapeutic Response.
    Article Snippet: Normal colon mucosal epithelial cell line NCM460 was purchased from the China Center for Type Culture Collection (Wuhan, China), and cells had been authenticated for STR profiling and tested for mycoplasma by the vendor.



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    normal colon mucosal epithelial cell line ncm460  (China Center for Type Culture Collection)
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    Functional analysis of the representative gene APCDD1 in IRPS ( A ) Comparison of the APCDD1 expression level between CRC tissues and normal control tissues based on the GEPIA2 database; ( B ) The expression level of APCDD1 in CRC lines SW620, HT29 and SW480 was detected by qRT-PCR with the normal colon epithelial cell line <t>NCM460</t> as a control; ( C ) The siRNA interference efficiency of APCDD1 in HT29 cell line was detected by qRT-PCR; ( D ) CCK-8 assays were used to detect the effect of APCDD1 knockdown on HT29 cell proliferation; ( E and F ) Transwell assays were used to detect the effect of APCDD1 knockdown on HT29 cell migration ( E ) and invasion ( F ); ( G ) Apoptosis assays were used to detect the effect of APCDD1 knockdown on apoptosis of HT29 cells. * P < 0.05; ** P < 0.01; *** P < 0.001
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    Functional analysis of the representative gene APCDD1 in IRPS ( A ) Comparison of the APCDD1 expression level between CRC tissues and normal control tissues based on the GEPIA2 database; ( B ) The expression level of APCDD1 in CRC lines SW620, HT29 and SW480 was detected by qRT-PCR with the normal colon epithelial cell line NCM460 as a control; ( C ) The siRNA interference efficiency of APCDD1 in HT29 cell line was detected by qRT-PCR; ( D ) CCK-8 assays were used to detect the effect of APCDD1 knockdown on HT29 cell proliferation; ( E and F ) Transwell assays were used to detect the effect of APCDD1 knockdown on HT29 cell migration ( E ) and invasion ( F ); ( G ) Apoptosis assays were used to detect the effect of APCDD1 knockdown on apoptosis of HT29 cells. * P < 0.05; ** P < 0.01; *** P < 0.001

    Journal: BMC Cancer

    Article Title: A novel machine learning-based immune prognostic signature for improving clinical outcomes and guiding therapy in colorectal cancer: an integrated bioinformatics and experimental study

    doi: 10.1186/s12885-025-13437-0

    Figure Lengend Snippet: Functional analysis of the representative gene APCDD1 in IRPS ( A ) Comparison of the APCDD1 expression level between CRC tissues and normal control tissues based on the GEPIA2 database; ( B ) The expression level of APCDD1 in CRC lines SW620, HT29 and SW480 was detected by qRT-PCR with the normal colon epithelial cell line NCM460 as a control; ( C ) The siRNA interference efficiency of APCDD1 in HT29 cell line was detected by qRT-PCR; ( D ) CCK-8 assays were used to detect the effect of APCDD1 knockdown on HT29 cell proliferation; ( E and F ) Transwell assays were used to detect the effect of APCDD1 knockdown on HT29 cell migration ( E ) and invasion ( F ); ( G ) Apoptosis assays were used to detect the effect of APCDD1 knockdown on apoptosis of HT29 cells. * P < 0.05; ** P < 0.01; *** P < 0.001

    Article Snippet: The human CRC cell lines HT29, SW480 and SW620, as well as the human normal colon mucosal epithelial cell line NCM460, were purchased from KeyGEN BioTECH Corp., Ltd (China).

    Techniques: Functional Assay, Comparison, Expressing, Control, Quantitative RT-PCR, CCK-8 Assay, Knockdown, Migration